Ring Finger Protein 115 (RNF115) antibody

Antigen: Ring Finger Protein 115 (RNF115)

Synonyms: BCA2, ZNF364

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185588

Additional Information

Alternative name: Rabring 7 / BCA2

Gene ID: 27246, 67845 (mouse), 362002 (rat)

Immunogen: C-DQDNRANERGHQTH

Format: Affinity-purified

Description: All names and symbols: Rabring 7, BCA2, ZNF364, zinc finger protein 364, CL469780, RNF115 , breast cancer-associated gene 2, ring finger protein 115. Official Symbol: RNF115. RefSeq number(s): NP_055270.1

Specificity: Human, Mouse, Rat, Dog 304 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in Human lysates of cell line HEPG2 (calculated MW of 33.7kDa according to NP_055270.1). Recommended concentration: 0.3-1µg/ml.

Concentration: 0.5 mg/ml

Purity: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185588 (0.3µg/ml) staining of HEPG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Burger, Gao, Amemiya et al.: "A novel RING-type ubiquitin ligase breast cancer-associated gene 2 correlates with outcome in invasive breast cancer." in: Cancer research, Vol. 65, Issue 22, pp. 10401-12, 2005 (PubMed).