Proline Dehydrogenase (Oxidase) 1 (PRODH) antibody

Antigen: Proline Dehydrogenase (Oxidase) 1 (PRODH)

Synonyms: POX, PIG6, SCZD4, HSPOX2, PRODH1, PRODH2, TP53I6, FLJ33744, MGC148078, MGC148079, PRODH, MGC140683, MGC115247, Pro1, Pro-1, Ym24d07, MGC159030, EE85, sl, slg, tu3, DmelCG1417, CG1417, ARABIDOPSIS THALIANA PROLINE OXIDASE, AT-POX, ATPDH, ATPOX, EARLY RESPONSIVE TO DEHYDRATION 5, ERD5, PRO1, PROLINE DEHYDROGENASE, PROLINE OXIDASE

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: ELISA

Catalog no.: ABIN185594

Additional Information

Alternative name: PRODH

Gene ID: 5625, 19125 (mouse)

Immunogen: C-ERDGSGTNKRDKQYQ

Description: All names and symbols: PRODH, proline dehydrogenase (oxidase) 1 , HSPOX2, PIG6, PRODH1, PRODH2, SCZD4, TP53I6, FLJ33744, MGC148078, MGC148079, Proline oxidase, mitochondrial, p53 induced protein, proline dehydrogenase (proline oxidase ), proline oxidase 2, tumor protein p53 inducible protein 6. Official Symbol: PRODH. RefSeq number(s): NP_057419.2

Specificity: Human, Mouse

Synonyms: POX, PIG6, SCZD4, HSPOX2, PRODH1, PRODH2, TP53I6, FLJ33744, MGC148078, MGC148079, PRODH, MGC140683, MGC115247, Pro1, Pro-1, Ym24d07, MGC159030, EE85, sl, slg, tu3, DmelCG1417, CG1417, ARABIDOPSIS THALIANA PROLINE OXIDASE, AT-POX, ATPDH, ATPOX, EARLY RESPONSIVE TO DEHYDRATION 5, ERD5, PRO1, PROLINE DEHYDROGENASE, PROLINE OXIDASE

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave bands at approx 85kDa and 25kDa in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 68.0kDa according to NP_057419.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Apoptosis/Necrosis, Metabolism

Restrictions: For Research Use only

Publications

Paterlini, Zakharenko, Lai et al.: "Transcriptional and behavioral interaction between 22q11.2 orthologs modulates schizophrenia-related phenotypes in mice." in: Nature neuroscience, Vol. 8, Issue 11, pp. 1586-94, 2005 (PubMed).