Prolactin-Inducible Protein (PIP) antibody

Antigen: Prolactin-Inducible Protein (PIP)

Synonyms: GPIP4, GCDFP15, GCDFP-15

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185621

Additional Information

Alternative name: GCDFP15 / PIP

Gene ID: 5304

Immunogen: C-GANKAQDNTRK

Format: Affinity-purified

Description: All names and symbols: PIP, GCDFP15, prolactin-induced protein , HGNC:8993, GCDFP-15, GP17, GPIP4, prolactin-inducible protein, gross cystic disease fluid protein 15. Official Symbol: PIP. RefSeq number(s): NP_002643.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185621 (0.3µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: OConnell, Wang, Odze: "Utility of immunohistochemistry in distinguishing primary adenocarcinomas from metastatic breast carcinomas in the gastrointestinal tract." in: Archives of pathology & laboratory medicine, Vol. 129, Issue 3, pp. 338-47, 2005 (PubMed).