Aldo-keto Reductase Family 1 Member C4 (AKR1C4) antibody

Antigen: Aldo-keto Reductase Family 1 Member C4 (AKR1C4)

Synonyms: C11, CDR, DD4, CHDR, HAKRA, MGC22581, 3-alpha-HSD

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185625

Additional Information

Alternative name: AKR1C4

Gene ID: 1109

Immunogen: Peptide with sequence DPKYQRVELNDGH-C, from the N Terminus of the protein sequence according to NP_001809.2

Format: Affinity-purified

Description: All names and symbols: AKR1C4, aldo-keto reductase family 1, member C4, HGNC:387, 3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581, 3-alpha hydroxysteroid dehydrogenase, type I , 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4, 3-alpha-hydroxysteroid dehydrogenase variant, aldo-keto reductase family 1, member C4, chlordecone reductase, dihydrodiol dehydrogenase 4, dihydrodiol dehydrogenase isozyme DD4, type I 3-alpha-hydroxysteroid dehydrogenase,. Official Symbol: AKR1C4. RefSeq number(s): NP_001809.2.

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx. 35kDa band observed in Human Liver lysates (calculated MW of 37.1kDa according to NP_001809.2). Recommended concentration: 0.1-0.3µg/ml. Immunohistochemistry: In paraffin embedded Human Liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Lord, Mack, Van Den Berg et al.: "Polymorphisms in genes involved in estrogen and progesterone metabolism and mammographic density changes in women randomized to postmenopausal hormone therapy: results from a pilot study." in: Breast cancer research : BCR, Vol. 7, Issue 3, pp. R336-44, 2005 (PubMed).