Amphiphysin (AMPH) antibody

Antigen: Amphiphysin (AMPH)

Synonyms: AMPH1, Amp, DAMP, amph, dAmph, DmelCG8604, CG8604, Amph1, MGC49429, MGC73193, zgc:73193, wu:fq25h04, MGC83546, AMPH, MGC140160, Amph, GB16263

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185628

Additional Information

Alternative name: Amphiphysin / AMPH

Gene ID: 273, 218038 (mouse)

Immunogen: Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1, NP_647477.1

Format: Affinity-purified

Description: All names and symbols: AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) , HGNC:471, AMPH1. Official Symbol: AMPH. RefSeq number(s): NP_001626.1, NP_647477.1.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct,8(10):2003-15., PMID: 9348539). Recommended concentration: 0.1-0.5µg/ml. A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Sommer, Weishaupt, Brinkhoff et al.: "Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin." in: Lancet, Vol. 365, Issue 9468, pp. 1406-11, 2005 (PubMed).