Neuroepithelial Cell Transforming 1 (NET1) (internal) antibody

Antigen: Neuroepithelial Cell Transforming 1 (NET1)

Synonyms: NET1A, ARHGEF8, Net1a, mNET1, AI604373, AU015857, 0610025H04Rik, 9530071N24Rik, MGC114559, MGC127767, net1a, xNET1, arhgef8, MGC53602, MGC145538, DKFZp459I0423, net1, NET1, zgc:92121, wu:fb13g03, wu:fb25c11

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185644

Additional Information

Alternative name: NET1 / ARHGEF8 (Internal)

Gene ID: 10276, 56349 (mouse), 307098 (rat)

Immunogen: C-DKLEYLDEKQRDPR

Format: Affinity-purified

Description: All names and symbols: NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator. Official Symbol: NET1. RefSeq number(s): NP_005854.2, NP_001040625.1

Specificity: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2). Human, Mouse, Rat, Dog 596aa, 542 aa

Synonyms: NET1A, ARHGEF8, Net1a, mNET1, AI604373, AU015857, 0610025H04Rik, 9530071N24Rik, MGC114559, MGC127767, net1a, xNET1, arhgef8, MGC53602, MGC145538, DKFZp459I0423, net1, NET1, zgc:92121, wu:fb13g03, wu:fb25c11

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185644 (2.5µg/ml) staining of paraffin embedded Human Breat. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Qin, Carr, Wu et al.: "Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1." in: The Journal of biological chemistry, Vol. 280, Issue 9, pp. 7603-13, 2005 (PubMed).