GRP94 antibody (Internal Region)

Catalog No. ABIN185655

The Goat Polyclonal anti-GRP94 antibody (ABIN185655) specifically detects GRP94 in WB, IHC, FACS, IF and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for GRP94 antibody (Internal Region) (ABIN185655)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Grade: Verified

Product Details anti-GRP94 Antibody

Binding Specificity: Internal Region

Purpose: GPR94 / TRA1

Sequence: KEGVKFDESE KTKE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5 kDa according to Human NP_003290.1 and Mouse NP_035761.1). This molecula

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: HSP90B1

Background: TRA1, GRP94, tumor rejection antigen (gp96) 1 , HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96), endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1

Gene ID: 7184, 22027, 362862

NCBI Accession: NP_003290

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades