Prion Protein (PRNP) (AA 143-153) antibody

Antigen: Prion Protein (PRNP)

Synonyms: CJD, GSS, PrP, ASCR, PRIP, PrPc, CD230, prion, MGC26679, PrP27-30, PrP33-35C, Prn, MGC140197, prp, Prn-p, cjd, prp(c), MGC84114, PRNP, PRP, Prp, SIP, PRPC, DKFZp459A016

Epitope: AA 143-153

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185661

Additional Information

Alternative name: Prion Protein (143-153)

Gene ID: 5621, 19122 (mouse), 24686 (rat)

Immunogen: C-SDYEDRYYREN

Format: Affinity-purified

Description: All names and symbols: PRNP , prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia) , ASCR, CD230, CJD, GSS, MGC26679, PRIP, PrP, PrP27-30, PrP33-35C, PrPc , CD230 antigen, major prion protein, prion protein, prion protein (p27-30) (Creutzfeld-Jakob disease Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia), prion protein PrP, prion-related protein . Official Symbol: PRNP . RefSeq number(s): NP_000302.1, NP_898902.1

Specificity: All variants represent the same protein. Human, Mouse, Rat, Dog, etc 253 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 25kDa band observed in Human Brain lysates (calculated MW of 27.7kDa according to NP_000302.1 and NP_898902.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185661 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Ballerini, Gourdain, Bachy et al.: "Functional implication of cellular prion protein in antigen-driven interactions between T cells and dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7254-62, 2006 (PubMed).