Wilms Tumor 1 (WT1) antibody

Antigen: Wilms Tumor 1 (WT1)

Synonyms: GUD, WAGR, WT33, WIT-2, WT1, WT, wt1-A, xWT1, CWT1, WT-1, wt1, MGC152697, zgc:152697, si:ch211-160j6.3, gud, wagr, wt-1, wt33, xwt1, wit-2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185674

Additional Information

Alternative name: Wilms tumor 1 / WT1

Gene ID: 7490

Immunogen: QDPASTCVPEPASQH

Format: Affinity-purified

Description: All names and symbols: WT1, Wilms tumor 1, GUD, WAGR, WIT-2, WT33 . Official Symbol: WT1. RefSeq number(s): NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3

Specificity: This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3). Human 497 aa, 514 aa, 500 aa, 517 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 55kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3kDa according to NP_000369.3). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185674 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Rao, Pham, Imam et al.: "Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis." in: Genes & development, Vol. 20, Issue 2, pp. 147-52, 2006 (PubMed).