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NLR Family, Pyrin Domain Containing 3 (NLRP3) (Internal Region) antibody

Antigen

NLR Family, Pyrin Domain Containing 3 (NLRP3)
Synonyms AII, AVP, FCU, MWS, FCAS, CIAS1, NALP3, C1orf7, CLR1.1, PYPAF1, AII/AVP, AGTAVPRL, FLJ95925, Cias1, Mmig1, Pypaf1, MGC129375, NLRP3, MGC159873, avp, cias1, nalp3, pypaf1, agtavprl
Binding Site
Alternatives

Internal Region
Clonality Polyclonal
Host
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA
1 reference available
Catalog no. ABIN185676
Quantity 100 ug
Price 261.61 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CIAS1 / Cryopyrin
Gene ID 114548
Immunogen C-DLYEKAKRDEPK
Description All names and symbols: CIAS1, cold autoinflammatory syndrome 1, AGTAVPRL, AII/AVP, C1orf7, FCAS, FCU, MWS, NALP3, PYPAF1 , AII/AVP receptor-like, Muckle-Wells syndrome, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, angiotensin/vasopressin receptor AII/AVP-like, cryopyrin. Official Symbol: CIAS1. RefSeq number(s): NP_004886.3, NP_899632.1
Specificity This antibody is expected to recognise both isoform a (NP_004886.3 and NP_001073289.1) and isoform b (NP_899632.1). Human, Dog

Application Details
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Application Notes Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Kanneganti, Ozoeren, Body-Malapel et al.: "Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3." in: Nature, Vol. 440, Issue 7081, pp. 233-6, 2006 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "NLR Family, Pyrin Domain Containing 3 (NLRP3)", type "Antibodies"
Hosts Rabbit (54), Goat (15), Mouse (11), Chicken (2), Sheep (2)
Reactivities Human (71), Mouse (Murine) (40), Rat (Rattus) (37), Dog (Canine) (19), Horse (Equine) (18), Cow (Bovine) (2), Cat (Feline) (1), Chicken (1)
Applications Immunofluorescence (IF) (36), Western Blotting (WB) (36), ELISA (32), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoprecipitation (IP) (6), Enzyme Immunoassay (EIA) (4), Immunocytochemistry (ICC) (4), Radioimmunoassay (RIA) (4), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1)
Conjugates Biotin (5), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes C-Term (6), N-Term (6), AA 1-50 (2), AA 1-93 (2), Internal Region (2), AA 4-223 (1), AA 84-101 (1)