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NLR Family, Pyrin Domain Containing 3 (NLRP3) (Internal Region) antibody

Details for Product No. ABIN185676
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Antigen
Synonyms CIAS1, Nalp3, avp, cias1, nalp3, pypaf1, agtavprl, AGTAVPRL, AII, AVP, C1orf7, CLR1.1, FCAS, FCU, MWS, NALP3, PYPAF1, AII/AVP, Cias1, Mmig1, Pypaf1
Epitope
Internal Region
(23), (8), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(93), (37), (30), (16), (5), (2)
Host
Goat
(59), (19), (16), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(63), (39), (21), (20), (14), (7), (7), (5), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185676
261.61 $
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Immunogen Peptide with sequence C-DLYEKAKRDEPK, from the internal region of the protein sequence according to NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1.
Specificity This antibody is expected to recognise all five reported isoforms (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CIAS1 / Cryopyrin
Background Synonyms: CIAS1, cold autoinflammatory syndrome 1, AGTAVPRL, AII/AVP, C1orf7, FCAS, FCU, MWS, NALP3, PYPAF1, AII/AVP receptor-like, Muckle-Wells syndrome, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, angiotensin/vasopressin receptor AII/AVP-like, cryopyrin
Gene ID 114548
NCBI Accession NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 70 kDa band in human lymph node and tonsil lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118 kDa according to NP_004886.3 and 106 kDa according to NP_899632.1. The 70 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Kanneganti, Ozören, Body-Malapel et al.: "Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3." in: Nature, Vol. 440, Issue 7081, pp. 233-6, 2006 (PubMed).

Validation Images
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