NLRP3 antibody (N-Term)
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- Target See all NLRP3 Antibodies
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
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Binding Specificity
- AA 12-31, N-Term
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NLRP3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for NACHT, LRR and PYD domains-containing protein 3(NLRP3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- RYLEDLEDVD LKKFKMHLED
- Cross-Reactivity (Details)
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Characteristics
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Rabbit IgG polyclonal antibody for NACHT, LRR and PYD domains-containing protein 3(NLRP3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: NLR family, pyrin domain containing 3
Protein Name: NACHT, LRR and PYD domains-containing protein 3 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.
- Isotype
- IgG
- Top Product
- Discover our top product NLRP3 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." in: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).
: "Alcohol intake aggravates adipose browning and muscle atrophy in cancer-associated cachexia." in: Oncotarget, Vol. 8, Issue 59, pp. 100411-100420, (2017) (PubMed).
: "Maternal obesity induces gut inflammation and impairs gut epithelial barrier function in nonobese diabetic mice." in: The Journal of nutritional biochemistry, Vol. 25, Issue 7, pp. 758-64, (2014) (PubMed).
: "Grape seed extract prevents skeletal muscle wasting in interleukin 10 knockout mice." in: BMC complementary and alternative medicine, Vol. 14, pp. 162, (2014) (PubMed).
: "
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Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." in: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).
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- Target
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
- Alternative Name
- NLRP3 (NLRP3 Products)
- Synonyms
- CIAS1 antibody, Nalp3 antibody, avp antibody, cias1 antibody, nalp3 antibody, pypaf1 antibody, agtavprl antibody, AGTAVPRL antibody, AII antibody, AVP antibody, C1orf7 antibody, CLR1.1 antibody, FCAS antibody, FCU antibody, MWS antibody, NALP3 antibody, PYPAF1 antibody, AII/AVP antibody, Cias1 antibody, Mmig1 antibody, Pypaf1 antibody, NLR family pyrin domain containing-like antibody, NLR family pyrin domain containing 3 antibody, NLR family, pyrin domain containing 3 L homeolog antibody, NLR family, pyrin domain containing 3 antibody, NLRPL antibody, NLRP3 antibody, nlrp3.L antibody, Nlrp3 antibody
- Background
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NLRP3(NLR FAMILY, PYRIN DOMAIN-CONTAINING 3), also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3(NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.
Synonyms: AGTAVPRL antibody|AII/AVP antibody|Angiotensin/vasopressin receptor AII/AVP like antibody|Angiotensin/vasopressin receptor AII/AVP-like antibody|C1orf7 antibody|Caterpiller protein 1.1 antibody|CIAS 1 antibody|CIAS1 antibody|CLR1.1 antibody|Cold autoinflammatory syndrome 1 antibody|Cold autoinflammatory syndrome 1 protein antibody|Cryopyrin antibody|Familial cold autoinflammatory syndrome antibody|FCAS antibody|FCU antibody|LRR and PYD domains-containing protein 3 antibody|Muckle-Wells syndrome antibody|MWS antibody|NACHT antibody|NACHT LRR and PYD containing protein 3 antibody|NALP 3 antibody|NALP3 antibody|NALP3_HUMAN antibody|NLRP3 antibody|PYPAF 1 antibody|PYPAF1 antibody|PYRIN containing APAF1 like protein 1 antibody|PYRIN-containing APAF1-like protein 1 antibody - UniProt
- Q96P20
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome
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