NLRP3 antibody (AA 15-120)

Catalog No. ABIN1386361

This Rabbit Polyclonal antibody specifically detects NLRP3 in WB, IF, IHC (p), IHC (fro) and IF (p). It exhibits reactivity toward Human. It has been mentioned in 11+ publications

Quick Overview for NLRP3 antibody (AA 15-120) (ABIN1386361)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-NLRP3 Antibody

Binding Specificity: AA 15-120

Purpose: NALP3/CIAS1 Polyclonal Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cryopyrin

Isotype: IgG

Application Details

Application Notes: WB(1:500-2000), IHC-P(1:100-500), IHC-F(1:100-500), IF(IHC-P)(1:100-500), IF(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NLRP3 antibody (ABIN1386361)

Li, Jiang, Ye, Zhou, Liu, Yang, Hao, Hu: "P2Y14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages." in: Cell death & disease, Vol. 11, Issue 5, pp. 394, (2021) (PubMed).

Yang, Cheng, Liu, Liu, Tan, Guan, Guo, Kuang: "Datura Metel L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8-MyD88-NF-κB-NLRP3 Inflammasome Pathway." in: Molecules (Basel, Switzerland), Vol. 24, Issue 11, (2019) (PubMed).

Zendedel, Mönnink, Hassanzadeh, Zaminy, Ansar, Habib, Slowik, Kipp, Beyer: "Estrogen Attenuates Local Inflammasome Expression and Activation after Spinal Cord Injury." in: Molecular neurobiology, (2017) (PubMed).

Yu, Liu, Sun, Wang, Wang, Zhao, Dai, Zhao, Zhang, Li, Han, Lu, Dong, Liu, Yu, Li: "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." in: Oncotarget, Vol. 8, Issue 23, pp. 37705-37716, (2017) (PubMed).

Zhang, Pan, Yang, Fu, Liu, Chi, Zhang, Hong, Ma, Yin: "H3 Relaxin Protects Against Myocardial Injury in Experimental Diabetic Cardiomyopathy by Inhibiting Myocardial Apoptosis, Fibrosis and Inflammation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 43, Issue 4, pp. 1311-1324, (2017) (PubMed).

Fernandez, Miller, Krammer, Gopal, Greenbaum, Bhardwaj: "Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells." in: Journal of leukocyte biology, Vol. 99, Issue 5, pp. 723-34, (2016) (PubMed).

Zhou, Rivera, Bhosle, Lahaie, Shao, Tahiri, Zhu, Polosa, Dorfman, Beaudry-Richard, Costantino, Lodygensky, Lachapelle, Chemtob: "Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β." in: The American journal of pathology, Vol. 186, Issue 12, pp. 3100-3116, (2016) (PubMed).

Debye, Schmülling, Zhou, Rune, Beyer, Johann: "Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1(G93A) ALS mice." in: Brain pathology (Zurich, Switzerland), (2016) (PubMed).

Heitzer, Kaiser, Kanagaratnam, Zendedel, Hartmann, Beyer, Johann: "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1(G93A) ALS Mice." in: Molecular neurobiology, (2016) (PubMed).

Zendedel, Johann, Mehrabi, Joghataei, Hassanzadeh, Kipp, Beyer: "Activation and Regulation of NLRP3 Inflammasome by Intrathecal Application of SDF-1a in a Spinal Cord Injury Model." in: Molecular neurobiology, (2015) (PubMed).

Johann, Heitzer, Kanagaratnam, Goswami, Rizo, Weis, Troost, Beyer: "NLRP3 inflammasome is expressed by astrocytes in the SOD1 mouse model of ALS and in human sporadic ALS patients." in: Glia, (2015) (PubMed).

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: CIAS1

Background: Synonyms: LRR and PYD domains-containing protein 3, AGTAVPRL, AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like, Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, Caterpiller protein 1.1, CIAS 1, CIAS1, CLR1.1, Cold autoinflammatory syndrome 1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, Familial cold autoinflammatory syndrome, FCAS, FCU, Muckle-Wells syndrome, MWS, NACHT, NACHT LRR and PYD containing protein 3, NALP 3, NALP3, NALP3_HUMAN, NLRP3, PYPAF 1, PYPAF1 antibody PYRIN containing APAF1 like protein 1, PYRIN-containing APAF1-like protein 1.

Gene ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome