Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP) antibody

Antigen: Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP)

Synonyms: CILP-1, HsT18872, 9830114D16, C130036G17Rik, CILP, NTPPH, im:7137994

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185679

Additional Information

Alternative name: CILP

Gene ID: 8483, 214425 (mouse), 315761 (rat)

Immunogen: C-NYRRTDHEDPRVK

Format: Affinity-purified

Description: All names and symbols: CILP, cartilage intermediate layer protein, nucleotide pyrophosphohydrolase , HGNC:1980, HsT18872 , cartilage intermediate layer protein. Official Symbol: CILP. RefSeq number(s): NP_003604.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 150kDa band observed in rat spinal cord lysates (calculated MW of 133kDa according to human NP_003604.2 and of 132kDa according to Rat NP_001101631.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Extracellular Matrix

Restrictions: For Research Use only

Images

ABIN185679 (0.03µg/ml) staining of rat spinal cord lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Seki, Kawaguchi, Chiba et al.: "A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease." in: Nature genetics, Vol. 37, Issue 6, pp. 607-12, 2005 (PubMed).