GTF2I Repeat Domain Containing 1 (GTF2IRD1) antibody

Antigen: GTF2I Repeat Domain Containing 1 (GTF2IRD1)

Synonyms: BEN, WBS, GTF3, RBAP2, CREAM1, MUSTRD1, WBSCR11, WBSCR12, hMusTRD1alpha1, ESTM9, Cream1, Gtf2il, X83320, MusTRD1, MGC102438, 1700012P16Rik, Tg(Alb1-Myc)166.8Sst, Gtf3, GTF2IRD1

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185681

Additional Information

Alternative name: GTF2IRD1

Gene ID: 9569, 57080 (mouse), 246770 (rat)

Immunogen: C-NKFTKDTTKLEPAS

Description: All names and symbols: GTF2IRD1, GTF2I repeat domain containing 1 , HGNC:4661, CREAM1, GTF3, MUSTRD1, RBAP2, WBSCR11, WBSCR12, hMusTRD1alpha1 , GTF2I repeat domain-containing 1, Williams-Beuren syndrome chromosome region 11, general transcription factor 3, muscle TFII-I repeat . Official Symbol: GTF2IRD1. RefSeq number(s): NP_057412.1, NP_005676.2

Specificity: This antibody is expected to recognise both reported isoforms (NP_057412.1 and NP_005676.2). Human, Mouse, Rat, Dog 959 aa, 944 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 30kDa and 40kDa in Human Heart lysate after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 106kDa according to NP_057412.1 and NP_005676.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors

Restrictions: For Research Use only

Publications

Tassabehji, Hammond, Karmiloff-Smith et al.: "GTF2IRD1 in craniofacial development of humans and mice." in: Science (New York, N.Y.), Vol. 310, Issue 5751, pp. 1184-7, 2005 (PubMed).