Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1) antibody

Antigen: Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1)

Synonyms: apbb1, MGC80654, APBB1, MGC139452

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185689

Additional Information

Alternative name: APBB1 / FE65

Gene ID: 322

Immunogen: C-GSLKPKRLGAHTP

Format: Affinity-purified

Description: All names and symbols: APBB1, FE65, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), MGC:9072, RIR , adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like protein. Official Symbol: APBB1. RefSeq number(s): NP_001155.1, NP_663722.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1). Human 710 aa, 708 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Approx 100kDa band observed in lysates of NIH/3T3 and HeLa (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7,273(32):20128-33., PMID: 9685356). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185689 (0.1µg/ml) staining of NIH/3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hoe, Magill, Guenette et al.: "FE65 interaction with the ApoE receptor ApoEr2." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24521-30, 2006 (PubMed).