Sorting Nexin 26 (SNX26) (N-Term) antibody

Antigen: Sorting Nexin 26 (SNX26)

Synonyms: TCGAP, FLJ39019, NOMA-GAP, SNX26, Snx26, AW324378, tcgap

Epitope: N-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185694

Additional Information

Alternative name: SNX26 / TCGAP (N Terminus)

Gene ID: 115703

Immunogen: ARSTDSLDGPGE-C

Format: Affinity-purified

Description: All names and symbols: SNX26, TCGAP, sorting nexin 26, FLJ39019. Official Symbol: SNX26. RefSeq number(s): NP_443180.2

Specificity: Human 1126 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 35kDa band in human brain (hippocampus) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining of secretory cells in the glands. Recommended concentration, 1.25µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185694 1.25µg/ml) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Liu, Nakazawa, Tezuka et al.: "Physical and functional interaction of Fyn tyrosine kinase with a brain-enriched Rho GTPase-activating protein TCGAP." in: The Journal of biological chemistry, Vol. 281, Issue 33, pp. 23611-9, 2006 (PubMed).