Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5) antibody

Antigen: Transcription Factor-Like 5 (Basic Helix-Loop-Helix) (TCFL5)

Synonyms: TCFL5, MGC137754, CHA, Figlb, E2BP-1, MGC46135, AV260129, MGC118431

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185720

Additional Information

Alternative name: CHA / TCFL5

Gene ID: 10732, 277353 (mouse)

Immunogen: C-KYIQERHGDSLKKE

Description: All names and symbols: TCFL5, CHA, transcription factor-like 5 (basic helix-loop-helix), E2BP-1, Figlb, MGC46135 , HPV-16 E2 binding protein 1, transcription factor-like 5 protein, Cha transcription factor. Official Symbol: TCFL5. RefSeq number(s): NP_006593.2

Specificity: Human, Mouse, Rat, Dog 500 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 55kDa band observed in Human Testis lysates (calculated MW of 52.7kDa according to NP_006593.2). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was greatly reduced by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185720 (0.3µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Siep, Sleddens-Linkels, Mulders et al.: "Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis." in: Nucleic acids research, Vol. 32, Issue 21, pp. 6425-36, 2004 (PubMed).