Lipin 1 (LPIN1) (internal) antibody

Antigen: Lipin 1 (LPIN1)

Synonyms: PAP1, KIAA0188, DKFZp781P1796, fld, Lipin1, Kiaa0188, mKIAA0188, 4631420P06, LPIN1, MGC146217

Epitope: internal

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN185721

Additional Information

Alternative name: Lipin 1 (Internal)

Gene ID: 23175, 14245 (mouse)

Immunogen: C-NKTDSPSRKRDK

Format: Affinity-purified

Description: All names and symbols: LPIN1, lipin 1 , DKFZp781P1796, KIAA0188. Official Symbol: LPIN1. RefSeq number(s): NP_663731.1

Specificity: Human, Mouse, Dog 890 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:23,000. Western Blot: Preliminary experiments gave bands at approx 50kDa and 26kDa in Human Adipose lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.7kDa according to NP_663731.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Suviolahti, Reue, Cantor et al.: "Cross-species analyses implicate Lipin 1 involvement in human glucose metabolism." in: Human molecular genetics, Vol. 15, Issue 3, pp. 377-86, 2006 (PubMed).