Homeobox B13 (HOXB13) antibody

Antigen: Homeobox B13 (HOXB13)

Synonyms: PSGD, HOXB13

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185732

Additional Information

Alternative name: Homeobox B13

Gene ID: 10481

Immunogen: C-KKVLAKVKNSATP

Format: Affinity-purified

Description: All names and symbols: homeobox B13, HOXB13, PSGD, homeo box B13. Official Symbol: HOXB13. RefSeq number(s): NP_006352.2. Gene ontology terms: fetal skin development and cutaneous regeneration

Specificity: Human 284 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 28kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 30.7kDa according to NP_006352.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185732 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Zhao, Yamashita, Ishikawa: "Regulation of tumor invasion by HOXB13 gene overexpressed in human endometrial cancer." in: Oncology reports, Vol. 13, Issue 4, pp. 721-6, 2005 (PubMed).