Intercellular Adhesion Molecule 5 (ICAM5) antibody

Antigen: Intercellular Adhesion Molecule 5 (ICAM5)

Synonyms: TLN, TLCN, CD50, Tlcn, Icam3, ICAM-3, MGC130520, ICAM5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185736

Additional Information

Alternative name: ICAM5 precursor

Gene ID: 7087, 15898 (mouse), 313785 (rat)

Immunogen: C-ESPAEGEVFAIQ

Format: Affinity-purified

Description: All names and symbols: ICAM5, intercellular adhesion molecule 5, telencephalin , TLCN, TLN , intercellular adhesion molecule 5, telencephalin. Official Symbol: ICAM5. RefSeq number(s): NP_003250.2

Specificity: Human, Mouse, Rat 924 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2kDa according to NP_003250.2. The 200-250kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185736 (2.5µg/ml) staining of paraffin embedded Human Brain (Cerebral Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Yoshihara, Oka, Nemoto et al.: "An ICAM-related neuronal glycoprotein, telencephalin, with brain segment-specific expression." in: Neuron, Vol. 12, Issue 3, pp. 541-53, 1994 (PubMed).