Zygin I (FEZ1) antibody

Antigen: Zygin I (FEZ1)

Synonyms: UNC76, UNC-76, MGC19293

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185737

Additional Information

Alternative name: FEZ1 / Zygin 1

Gene ID: 9638, 235180 (mouse), 81730 (rat)

Immunogen: C-QNKQKEQRELMKK

Format: Affinity-purified

Description: All names and symbols: FEZ1, zygin 1, fasciculation and elongation protein zeta 1 (zygin I). Official Symbol: FEZ1. RefSeq number(s): NP_005094.1

Specificity: This antibody is expected to recognize isoform 1 (NP_005094.1) only. Human, Mouse, Rat, Dog 392 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 45kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 45.1kDa according to NP_005094.1). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185737 (0.03µg/ml) staining of Human Brain (frontal Cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Suzuki, Okada, Semba et al.: "Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24948-56, 2005 (PubMed).