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Product Details

anti-Thymine Dimer antibody

Overview

Antigen: Thymine Dimer
Clonality: Monoclonal (KTM53)
Application: In situ hybridization (ISH)
Host: Mouse
6 references available 6 references available
Quantity: 0.1 mg
Price: 735,66 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN187263
Availability: Ships within 7 to 10 Business Days
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Additional Information  » anti-Thymine Dimer antibody

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Antigen Thymine Dimer
Immunogen UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.
Clonality Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Clone KTM53
Host Mouse
Specificity Reacts specifically with thymine dimers produced by UV irradiation in double- or single-stranded DNA. Does not react with (6-4) photo products.

Application Details  » anti-Thymine Dimer antibody

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Application In situ hybridization (ISH)
Application Notes In situ hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern and Dot Blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes used for in situ. (reported to work, but no data is available) Immunocytochemistry: Reported to work by end-user, protocol available. Immunohistochemistry: Published by user. The optimal dilution for a specific application should be determined by the researcher.
Buffer 200 µL of 0.5 mg/mL purified monoclonal antibody in PBS with protein stabilizer and 0.1% sodium azide. Purified by Protein A chromatography.
Storage Store at 4 °C for short term, store at -20 °C for long term. Avoid repeat freeze/thaw cycles.
Research Area DNA/RNA, Cancer
Restrictions For Research Use only

Publications  » anti-Thymine Dimer antibody

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Publications Ke, Camouse, Swain et al.: "UV protective effects of DNA repair enzymes and RNA lotion." In: Photochemistry and photobiology , Vol. 84, Issue 1, pp. 180-4, 2008 (PubMed)

Gambichler, Schlaffke, Tomi et al.: "Tacrolimus ointment neither blocks ultraviolet B nor affects expression of thymine dimers and p53 in human skin." In: Journal of dermatological science , Vol. 50, Issue 2, pp. 115-22, 2008 (PubMed)

Ellison, Smith, Gilliam et al.: "Inactivation of the vitamin D receptor enhances susceptibility of murine skin to UV-induced tumorigenesis." In: The Journal of investigative dermatology , Vol. 128, Issue 10, pp. 2508-17, 2008 (PubMed)

Batista, Roos, Kaina et al.: "p53 mutant human glioma cells are sensitive to UV-C-induced apoptosis due to impaired cyclobutane pyrimidine dimer removal." In: Molecular cancer research : MCR , Vol. 7, Issue 2, pp. 237-46, 2009 (PubMed)

da Silva, Ropke, Miranda et al.: "Photoprotective effect of Pothomorphe umbellata on UVB radiation-induced biomarkers involved in carcinogenesis of hairless mouse epidermis." In: Cutaneous and ocular toxicology , Vol. 28, Issue 2, pp. 54-60, 2009 (PubMed)

Bacqueville, Mavon: "Comparative analysis of solar radiation-induced cellular damage between ex vivo porcine skin organ culture and in vitro reconstructed human epidermis." In: International journal of cosmetic science , 2009 (PubMed)