Thymine Dimer antibody

Antigen: Thymine Dimer

Clonality: Monoclonal (KTM53)

Host: Mouse

Application: In situ hybridization (ISH)

Catalog no.: ABIN187263

Additional Information

Immunogen: UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.

Isotype: IgG1  (Matching secondary antibodies)

Clone: KTM53

Specificity: Reacts specifically with thymine dimers produced by UV irradiation in double- or single-stranded DNA. Does not react with (6-4) photo products.

Application Details

Application Notes: In situ hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern and Dot Blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes used for in situ. (reported to work, but no data is available) Immunocytochemistry: Reported to work by end-user, protocol available. Immunohistochemistry: Published by user. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 200 µL of 0.5 mg/mL purified monoclonal antibody in PBS with protein stabilizer and 0.1% sodium azide. Purified by Protein A chromatography.

Storage: Store at 4 °C for short term, store at -20 °C for long term. Avoid repeat freeze/thaw cycles.

Research Area: DNA/RNA, Cancer

Restrictions: For Research Use only

Publications

Publications: Ke, Camouse, Swain et al.: "UV protective effects of DNA repair enzymes and RNA lotion." in: Photochemistry and photobiology, Vol. 84, Issue 1, pp. 180-4, 2008 (PubMed).

Gambichler, Schlaffke, Tomi et al.: "Tacrolimus ointment neither blocks ultraviolet B nor affects expression of thymine dimers and p53 in human skin." in: Journal of dermatological science, Vol. 50, Issue 2, pp. 115-22, 2008 (PubMed).

Ellison, Smith, Gilliam et al.: "Inactivation of the vitamin D receptor enhances susceptibility of murine skin to UV-induced tumorigenesis." in: The Journal of investigative dermatology, Vol. 128, Issue 10, pp. 2508-17, 2008 (PubMed).

Batista, Roos, Kaina et al.: "p53 mutant human glioma cells are sensitive to UV-C-induced apoptosis due to impaired cyclobutane pyrimidine dimer removal." in: Molecular cancer research : MCR, Vol. 7, Issue 2, pp. 237-46, 2009 (PubMed).

da Silva, Ropke, Miranda et al.: "Photoprotective effect of Pothomorphe umbellata on UVB radiation-induced biomarkers involved in carcinogenesis of hairless mouse epidermis." in: Cutaneous and ocular toxicology, Vol. 28, Issue 2, pp. 54-60, 2009 (PubMed).

Bacqueville, Mavon: "Comparative analysis of solar radiation-induced cellular damage between ex vivo porcine skin organ culture and in vitro reconstructed human epidermis." in: International journal of cosmetic science, 2009 (PubMed).