Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
Species Reactivity: Human, other species not tested.
Immunogen
A BALB/c mouse was immunized with 5 µg of affinity purified nuclear extract proteins. Spleen cells were fused with equal number of Sp0Ag-14 myeloma cells. Cells whose supernatant showed a positive signal for a 104 kDa band were selected and cloned by limited dilution.
Clone 1027 can be applied for the detection of MVP/LRP in a large number of eukaryotic cells including the MCF-7 and Hela tumor cell lines. Immunohistochemistry: Frozen sections Western blot The optimal dilution for a specific application should be determined by the researcher.
LRP antibody, VAULT1 antibody, 2310009M24Rik antibody, cb771 antibody, wu:fb52b05 antibody, wu:fc02g01 antibody, mvp antibody, MGC145641 antibody, Tb05.45E22.810 antibody, major vault protein antibody, major vault protein L homeolog antibody, putative major vault protein antibody, MVP antibody, Mvp antibody, mvp antibody, mvp.L antibody, Tc00.1047053510353.10 antibody, Tb927.5.4460 antibody, Tb10.70.0520 antibody, Tb10.70.5840 antibody, LMJF_05_0060 antibody
Background
The mAb clone 1027 is specific for a 104 kDa protein. The antibody is one of four mAb which recognize different epitopes of the protein. This 104 kDa protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has been shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, protein and RNA composition of this complex the particles are different from other ribonucleoproteins. The mAb is especially applicable for studying the association of estrgen receptor with vaults and the study of the mechanism of action of estrogenic hormones.