Lead antibody
Quick Overview for Lead antibody (ABIN190340)
Target
Host
Clonality
Application
Clone
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Purpose
- Anti-Lead Mouse Monoclonal Antibody
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Specificity
- This antibody detects lead in the ppb range. Indirect ELISA endpoint titer is >1: 100,000.
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Purification
- Whole Antiserum
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Immunogen
- Lead conjugated to keyhole limpet hemocyanin (KLH) by proprietary chemistry.
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Isotype
- IgM
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Application Notes
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Indirect ELISA: optimized for lead-RSA (rabbit serum albumin) on solid phase at 200 ng/well. Ascites diluted 1:100,000 produces an O.D. signal of 0.5.
Competition ELISA: with lead-RSA on the solid phase at 200 ng/well, 50 % inhibition of antibody binding is achieved with Pb2+ at 800 ng/well.
These are recommended concentrations.
End users should determine optimal concentrations for their applications.Not all applications have been investigated. -
Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Dilute in PBS or medium that is identical to that used in the assay system.
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Concentration
- Lot specific
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Buffer
- Whole antiserum
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Storage
- -20 °C
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Storage Comment
- This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
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- Lead
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Target Type
- Chemical
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Background
- Exposure to potentially neurotoxic levels of lead occurs in approximately 9 % of American children under 6 years of age, and many urban dwellings still contain dangerous levels of lead paint.
Target
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