Fumarate Hydratase (FH) antibody

Antigen: Fumarate Hydratase (FH)

Synonyms: FH, HF1, MGC140701, Fh1, zf-e152, MGC66253, zgc:66253, zgc:77498, ns:zf-e152, DKFZp469O0739, Fh-1, MCL, LRCC, HLRCC, MCUL1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190758

Additional Information

Alternative name: Fumarase / FH

Gene ID: 2271, 14194 (mouse), 24368 (rat)

Immunogen: C-HPNDHVNKSQSSND

Format: Affinity-purified

Description: All names and symbols: fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata. Official Symbol: FH. RefSeq number(s): NP_000134.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190758 (0.01µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Fumarase (RC200614) and probed with ABIN190758 (mock transfection in first lane), tested by Origene.

Publications

Publications: Pollard, Spencer-Dene, Shukla et al.: "Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway." in: Cancer cell, Vol. 11, Issue 4, pp. 311-9, 2007 (PubMed).