RER1 Retention in Endoplasmic Reticulum 1 Homolog (S. Cerevisiae) (RER1) antibody

Antigen: RER1 Retention in Endoplasmic Reticulum 1 Homolog (S. Cerevisiae) (RER1)

Synonyms: RP4-740C4.2, AU043380, 1110060F11Rik, 5830454N22Rik, MGC116413, RGD1306324, MGC159658, MGC179436, RER1, DKFZp459K116, rer1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190761

Additional Information

Alternative name: RER1

Gene ID: 11079, 67830 (mouse)

Immunogen: C-HGKRRYRGKEDAGK

Format: Affinity-purified

Description: All names and symbols: RER1, RER1 retention in endoplasmic reticulum 1 homolog (S. cerevisiae), RP4-740C4.2, RER1 homolog, RER1 retention in endoplasmic reticulum 1. Official Symbol: RER1. RefSeq number(s): NP_008964.3

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.9kDa according to NP_008964.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Publications

Publications: Spasic, Raemaekers, Dillen et al.: "Rer1p competes with APH-1 for binding to nicastrin and regulates gamma-secretase complex assembly in the early secretory pathway." in: The Journal of cell biology, Vol. 176, Issue 5, pp. 629-40, 2007 (PubMed).

Kaether, Scheuermann, Fassler et al.: "Endoplasmic reticulum retention of the gamma-secretase complex component Pen2 by Rer1." in: EMBO reports, Vol. 8, Issue 8, pp. 743-8, 2007 (PubMed).