G6PD (AA 308-320) antibody
| Antigen |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
Western Blotting (WB) (45), Enzyme Immunoassay (EIA) (19), ELISA (18), Immunoprecipitation (IP) (10), Immunofluorescence (IF) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Dot Blot (Dot) (6), Immunodiffusion (ID) (6), Radioimmunoassay (RIA) (6), Immunohistochemistry (IHC) (5), Flow Cytometry (FACS) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectrophoresis (IEP) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN190766 |
| Quantity |
100µg (100µg/200µl) |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Gene ID
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2539, 14381 (mouse), 24377 (rat)
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Swiss-Prot
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NP_000393.4
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Immunogen
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Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 , NP_001035810.1.
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Cross-Reactivity
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Human, Mouse (Murine), Rat (Rattus), Dog (Canine)
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Format
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Liquid
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Grade
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Serum-free
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Description
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Official Symbol: G6PD
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Specificity
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This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1). Human, Mouse, Rat, Dog
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1µg/ml. IHC: In paraffin embedded Human Testis shows strong cytoplasm staining in spermatogonia. Recommended concentration, 2-4µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant G6PD captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
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Concentration
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100µg/200µl
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Guindo, Fairhurst, Doumbo et al.: "X-linked G6PD deficiency protects hemizygous males but not heterozygous females against severe malaria." in: PLoS medicine, Vol. 4, Issue 3, pp. e66, 2007 (PubMed).
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Alternatives for antigen "G6PD", type "Antibodies"
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Hosts
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Goat (22), Rabbit (17), Mouse (6)
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Reactivities
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Human (21), Leuconostoc mesenteroides (8), Dog (Canine) (5), Mouse (Murine) (4), Yeast (Saccharomyces Cerevisiae) (4), Rat (Rattus) (3), Bacteria (1), Yeast (1)
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Applications
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Western Blotting (WB) (45), Enzyme Immunoassay (EIA) (19), ELISA (18), Immunoprecipitation (IP) (10), Immunofluorescence (IF) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Dot Blot (Dot) (6), Immunodiffusion (ID) (6), Radioimmunoassay (RIA) (6), Immunohistochemistry (IHC) (5), Flow Cytometry (FACS) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectrophoresis (IEP) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (7), HRP (4)
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Epitopes
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AA 308-320 (2)
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