Podocalyxin-Like (PODXL) antibody

Antigen: Podocalyxin-Like (PODXL)

Synonyms: PC, PCLP, Gp200, MGC138240, Ly102, Pclp1, PCLP-1, AW121214, podocalyxin, PODXL, podxl, fc18d02, wu:fc18d02

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190772

Additional Information

Alternative name: PODXL

Gene ID: 5420, 27205 (mouse), 192181 (rat)

Immunogen: C-DNLTKDDLDEEEDTH

Format: Affinity-purified

Description: All names and symbols: PODXL, podocalyxin-like, Gp200, MGC138240, PCLP. Official Symbol: PODXL. RefSeq number(s): NP_001018121.1 , NP_005388.2

Specificity: This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Approx 160kDa band observed in Human Kidney and Mouse Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20,272(25):15708-14., PMID: 9188463). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cardiovascular, Hematopoietic Progenitors, Extracellular Matrix

Restrictions: For Research Use only

Images

ABIN190772 (0.5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Nielsen, Graves, Chelliah et al.: "The CD34-related molecule podocalyxin is a potent inducer of microvillus formation." in: PLoS ONE, Vol. 2, Issue 2, pp. e237, 2007 (PubMed).