Gabpb2 (mouse) antibody

Antigen: Gabpb2 (mouse)

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190777

Additional Information

Gene ID: 213054 (mouse)

Immunogen: C-KSGRTTEPHTN

Format: Affinity-purified

Description: All names and symbols: Gabpb2, GA binding protein transcription factor, beta 2. Official Symbol: Gabpb2. RefSeq number(s): NP_766100.1, NP_084161.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_766100.1, NP_084161.1). Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 35kDa band in Rat Kidney lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.7kDa according to NP_766100.1 and NP_084161.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: OLeary, Koleski, Kola et al.: "Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1." in: Gene, Vol. 344, pp. 79-92, 2005 (PubMed).