GA-Binding Protein Subunit beta-2 (GABPB2) antibody

Antigen: GA-Binding Protein Subunit beta-2 (GABPB2)

Synonyms: MGC29891, RP11-68I18.1, AV050852, Gabpb2-1, 5830427M07, 1810015F01Rik, 9430006E19Rik, A430024B14Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190778

Additional Information

Alternative name: GABPB2

Gene ID: 2553

Immunogen: C-SSENSSKATDETG

Format: Affinity-purified

Description: All names and symbols: GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53kDa, GA binding protein transcription factor, beta subunit 2, 47kDa, nuclear respiratory factor-2 subunit beta. Official Symbol: GABPB2. RefSeq number(s): NP_005245.2, NP_002032.2

Specificity: This antibody is expected to recognise isoform beta 1 (NP_005245.2) and isoform gamma 1 (NP_002032.2). Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 45+38kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5kDa according to NP_005245.2 and of 38.1kDa according to NP_002032.2). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 21kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN190778 (0.5µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: OLeary, Koleski, Kola et al.: "Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1." in: Gene, Vol. 344, pp. 79-92, 2005 (PubMed).