Non-SMC Condensin I Complex, Subunit H (NCAPH) antibody

Antigen: Non-SMC Condensin I Complex, Subunit H (NCAPH)

Synonyms: BRRN1, CAP-H, HCAP-H, NCAPH, MGC127159, MGC158618, si:dkeyp-86b9.4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190779

Additional Information

Alternative name: HCAP-H

Gene ID: 23397

Immunogen: C-TEDLSDVLVRQGD

Format: Affinity-purified

Description: All names and symbols: HCAP-H, NCAPH, non-SMC condensin I complex, subunit H, BRRN1, CAP-H, XCAP-H homolog, barren homolog 1, chromosome-associated protein H, condensin complex subunit 2. Official Symbol: NCAPH . RefSeq number(s): NP_056156.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Cell. 2001 Nov,12(11):3527-37., PMID: 11694586). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190779 (0.01µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Torres-Rosell, De Piccoli, Cordon-Preciado et al.: "Anaphase onset before complete DNA replication with intact checkpoint responses." in: Science (New York, N.Y.), Vol. 315, Issue 5817, pp. 1411-5, 2007 (PubMed).