Stromal Antigen 2 (STAG2) antibody

Antigen: Stromal Antigen 2 (STAG2)

Synonyms: SA2, SA-2, FLJ25871, bA517O1.1, DKFZp686P168, DKFZp781H1753, RGD1562042, STAG2, stag2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190782

Additional Information

Alternative name: STAG2

Gene ID: 10735, 20843 (mouse)

Immunogen: C-REPKRLRPEDS

Format: Affinity-purified

Description: All names and symbols: STAG2, stromal antigen 2, RP11-517O1.1, DKFZp686P168, DKFZp781H1753, FLJ25871, SA-2, SA2, bA517O1.1, SCC3 homolog 2. Official Symbol: STAG2 . RefSeq number(s): NP_001036214.1, NP_001036215.1 , NP_001036216.1 , NP_006594.3

Specificity: This antibody is expected to recognise isoform a (NP_001036214.1 and NP_001036215.1) and isoform b (NP_001036216.1 and NP_006594.3). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 146kDa according to NP_001036214.1 and NP_001036215.1). Recommended concentration: 0.01µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190782 (0.01µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Lara-Pezzi, Pezzi, Prieto et al.: "Evidence of a transcriptional co-activator function of cohesin STAG/SA/Scc3." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 6553-9, 2004 (PubMed).