Hap1 (mouse) antibody

Antigen: Hap1 (mouse)

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190785

Additional Information

Gene ID: 15114 (mouse), 29430 (rat)

Immunogen: C-QDAHSKRQQKQK

Format: Affinity-purified

Description: All names and symbols: Hap1, huntingtin-associated protein 1, RP23-392I3.11, HAP-1, MGC31449. Official Symbol: Hap1. RefSeq number(s): NP_034534.1

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 110kDa band in Mouse and Rat Brain lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.8kDa according to NP_034534.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Rong, McGuire, Fang et al.: "Regulation of intracellular trafficking of huntingtin-associated protein-1 is critical for TrkA protein levels and neurite outgrowth." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 22, pp. 6019-30, 2006 (PubMed).