Sal-Like 4 (Drosophila) (SALL4) antibody

Antigen: Sal-Like 4 (Drosophila) (SALL4)

Synonyms: SALL4, DRRS, HSAL4, ZNF797, MGC133050, dJ1112F19.1, Tex20, C78083, C78563, AA407717, AL022809, AW536104, 5730441M18Rik, C330011P20Rik, drrs, sall4a, znf797, MGC81541, hsal4, MGC76059, cb614, sb:cb372, sb:cb614, wu:fa01g03, ik:tdsubc_2c1, xx:tdsubc_2c1

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190787

Additional Information

Alternative name: Sall4 (mouse)

Gene ID: 99377 (mouse)

Immunogen: C-DQSKKGKGKPQN

Format: Affinity-purified

Description: All names and symbols: SALL4, sal-like 4 (Drosophila) , AA407717, AL022809, AW536104, C330011P20Rik, C78083, C78563, Tex20, sal-like 4. Official Symbol: Sall4. RefSeq number(s): NP_780512.2, NP_958797.2, NP_958798.2

Specificity: This antibody is expected to recognize isoform a (NP_780512.2) and isoform b (NP_958797.2). Mouse, Rat

Synonyms: SALL4, DRRS, HSAL4, ZNF797, MGC133050, dJ1112F19.1, Tex20, C78083, C78563, AA407717, AL022809, AW536104, 5730441M18Rik, C330011P20Rik, drrs, sall4a, znf797, MGC81541, hsal4, MGC76059, cb614, sb:cb372, sb:cb614, wu:fa01g03, ik:tdsubc_2c1, xx:tdsubc_2c1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70kDa band observed in Rat Spleen and Testis lysates (calculated MW of 66.2kDa according to NP_958797.2). Recommended concentration: 0.1-0.3µg/ml. Additional bands of unknown identity was also consistently observed at 28+24kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190787 (0.1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Wang, Rao, Chu et al.: "A protein interaction network for pluripotency of embryonic stem cells." in: Nature, Vol. 444, Issue 7117, pp. 364-8, 2006 (PubMed).