Interferon gamma Up-Regulated I-5111 Protein (PSME1) (isoform 1) antibody

Antigen: Interferon gamma Up-Regulated I-5111 Protein (PSME1)

Synonyms: PA28A, IFI5111, MGC8628, REGalpha, PA28alpha

Epitope: isoform 1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190798

Additional Information

Alternative name: PSME1 (isoform 1)

Gene ID: 5720, 19186 (mouse), 29630 (rat)

Immunogen: C-KNFEKLKKPRGETK

Format: Affinity-purified

Description: All names and symbols: PSME1, proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), IFI5111, MGC8628, PA28A, PA28alpha, REGalpha , 11S regulator complex alpha subunit, 29-kD MCP activator subunit, activator of multicatalytic protease subunit 1, interferon gamma up-regulated I-5111 protein, interferon-gamma IEF SSP 5111, interferon-gamma-inducible protein 5111, proteasome activator subunit 1, proteasome activator subunit-1. Official Symbol: PSME1. RefSeq number(s): NP_006254.1

Specificity: This antibody is expected to recognise isoform 1 (NP_006254.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 30kDa band observed in Human Lymph Node, Spleen, Tonsil and Peripheral Blood Mononuceocyte lysates (calculated MW of 28.7kDa according to NP_006254.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190798 (0.3µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Brychcy, Kuckelkorn, Hausdorf et al.: "Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28." in: Arthritis and rheumatism, Vol. 54, Issue 7, pp. 2175-83, 2006 (PubMed).