Chromodomain Helicase DNA Binding Protein 5 (CHD5) antibody

Antigen: Chromodomain Helicase DNA Binding Protein 5 (CHD5)

Synonyms: KIAA0444, DKFZp434N231, AW060752, 4930532L22Rik, B230399N07Rik, CHD5

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190809

Additional Information

Alternative name: CHD5

Gene ID: 26038, 269610 (mouse)

Immunogen: C-QSRRQLKSDRDKP

Format: Affinity-purified

Description: All names and symbols: CHD5, chromodomain helicase DNA binding protein 5 , DKFZp434N231, KIAA0444. Official Symbol: CHD5. RefSeq number(s): NP_056372.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 90kDa band in Human Brain (Cerebellum and Frontal Cortex) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 223kDa according to NP_056372.1. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Thompson, Gotoh, Kok et al.: "CHD5, a new member of the chromodomain gene family, is preferentially expressed in the nervous system." in: Oncogene, Vol. 22, Issue 7, pp. 1002-11, 2003 (PubMed).