Perilipin 1 (PLIN1) (internal) antibody

Antigen: Perilipin 1 (PLIN1)

Synonyms: PERI, PLIN, PERIA, Plin1, Peri, Plin, 6030432J05Rik, PLIN1, MGC138955, peri, plin, perilipin, LOC692833

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190818

Additional Information

Alternative name: Perilipin (internal)

Gene ID: 5346

Immunogen: C-PREKPKRRVSDS

Format: Affinity-purified

Description: All names and symbols: PLIN, perilipin, PERI, lipid droplet-associated protein. Official Symbol: PLIN. RefSeq number(s): NP_002657.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.01 µg/ml. Immunofluorescence: This product is not suitable for IF but the related product ABIN190817 is tested in this application.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190818 (0.01µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Wolins, Brasaemle, Bickel: "A proposed model of fat packaging by exchangeable lipid droplet proteins." in: FEBS letters, Vol. 580, Issue 23, pp. 5484-91, 2006 (PubMed).