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Nanog Homeobox Pseudogene 8 (NANOGP8) (C-Term) antibody

Antigen

Nanog Homeobox Pseudogene 8 (NANOGP8)
Synonyms ENK, ecat4, 2410002E02Rik, PN8, NANOG, NANOGP1, MGC119250, nanog
Epitope
Alternatives

C-Term
Clonality Polyclonal
Host
Reactivity
Alternatives

Human
Application
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
1 reference available
Catalog no. ABIN190835
Quantity 100µg  (100µg/200µl)
Price 501.80 $   Plus shipping costs $35.00
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anti-Nanog Homeobox Pseudogene 8 (NANOGP8) (C-Term) antibody

Additional Information
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Alternative name NANOG
Gene ID 79923
Swiss-Prot NP_079141.2
Immunogen Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.
Cross-Reactivity Human
Format Liquid
Grade Serum-free
Description Official Symbol: NANOG
Specificity Human

Application Details
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Application Notes Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1µg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources. IHC: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2µg/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Concentration 100µg/200µl
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications
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Publications Yasuda, Tsuneyoshi, Sumi et al.: "NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 11, Issue 9, pp. 1115-23, 2006 (PubMed).

Alternatives
Application Details Publications Images Secondary Antibodies back to top

Alternatives for antigen "Nanog Homeobox Pseudogene 8 (NANOGP8)", type "Antibodies"
Hosts Rabbit (84), Mouse (24), Goat (8)
Reactivities Human (110), Mouse (Murine) (31), Rat (Rattus) (24), Dog (Canine) (5), Chicken (3), Cow (Bovine) (3), Rabbit (2), Zebrafish (Brachydanio rerio) (2), Goat (1), Horse (Equine) (1), Pig (Porcine) (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (80), Immunofluorescence (IF) (51), ELISA (37), Flow Cytometry (FACS) (37), Enzyme Immunoassay (EIA) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
Conjugates Biotin (7), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
Epitopes C-Term (3), Center (3), N-Term (2), S285 (2), S71 (1)