Sorbin and SH3 Domain Containing 1 (SORBS1) (Internal Region) antibody

Antigen: Sorbin and SH3 Domain Containing 1 (SORBS1)

Synonyms: CAP, FLAF2, R85FL, SH3D5, SORB1, SH3P12, FLJ12406, KIAA1296, DKFZp451C066, DKFZp586P1422, Sh3d5, mKIAA1296, 2310065E01Rik, 9530001P15Rik

Binding Site: Internal Region

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN190836

Additional Information

Alternative name: SORBS1/ponsin

Gene ID: 10580, 20411 (mouse)

Immunogen: C-SERNDWEPPDKKVD

Description: All names and symbols: SORBS1, sorbin and SH3 domain containing 1, CAP, DKFZp451C066, DKFZp586P1422, FLAF2, FLJ12406, KIAA1296, R85FL, SH3D5, SH3P12, SORB1 , Fas-ligand associated factor 2, SH3-domain protein 5, SH3-domain protein 5 (ponsin), c-Cbl associated protein, ponsin. Official Symbol: SORBS1. RefSeq number(s): NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1, NP_001030129.1

Specificity: This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1) Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6kDa according to NP_006425.2 and higher sizes for other isoforms. The 65kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Baumann, Ribon, Kanzaki et al.: "CAP defines a second signalling pathway required for insulin-stimulated glucose transport." in: Nature, Vol. 407, Issue 6801, pp. 202-7, 2000 (PubMed).