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Sorbin and SH3 Domain Containing 1 (SORBS1) (Internal Region) antibody

Details for Product No. ABIN190836, Supplier: Login to see
Antigen
  • CAP
  • FLAF2
  • R85FL
  • SH3D5
  • SH3P12
  • SORB1
  • 2310065E01Rik
  • 9530001P15Rik
  • Sh3d5
  • mKIAA1296
Epitope
Internal Region
41
20
16
10
2
1
1
1
Reactivity
Human, Mouse (Murine)
75
12
8
5
4
3
3
2
1
1
Host
Goat
54
24
1
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
5
5
5
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
65
25
20
11
8
6
1
1
Supplier
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Immunogen Peptide with sequence C-SERNDWEPPDKKVD, from the internal region of the protein sequence according to NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1, NP_001030129.1.
Blocking Peptide Blocking peptide for this product available: ABIN369864
Specificity This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SORBS1/ponsin (SORBS1 Antibody Abstract)
Background Synonyms: SORBS1, sorbin and SH3 domain containing 1, CAP, DKFZp451C066, DKFZp586P1422, FLAF2, FLJ12406, KIAA1296, R85FL, SH3D5, SH3P12, SORB1, Fas-ligand associated factor 2, SH3-domain protein 5, SH3-domain protein 5 (ponsin), c-Cbl associated protein, ponsin
Gene ID 10580, 20411
NCBI Accession NP_001030127, NP_001030126, NP_001030129, NP_001030128, NP_056200, NP_079267, NP_006425
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 65 kDa band in Human Adipose lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6 kDa according to NP_006425.2 and higher sizes for other isoforms. The 65 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Baumann, Ribon, Kanzaki et al.: "CAP defines a second signalling pathway required for insulin-stimulated glucose transport." in: Nature, Vol. 407, Issue 6801, pp. 202-7, 2000 (PubMed).