Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1) antibody

Antigen: Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1)

Synonyms: P4HA, P4ha, AL022634, PHalphaI, P4HA2, P4HA1, DKFZp468D054

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190847

Additional Information

Alternative name: P4HA1

Gene ID: 5033, 18451 (mouse), 64475 (rat)

Immunogen: C-DFARKDEPDAFKE

Format: Affinity-purified

Description: All names and symbols: P4HA1, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I, 4-PH alpha-1, P4HA , prolyl 4-hydroxylase, alpha I subunit. Official Symbol: P4HA1. RefSeq number(s): NP_000908.2, NP_001017962.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in lysates of cell lines HEK293, A431 and NIH3T3 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1µg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Extracellular Matrix, Organelles

Restrictions: For Research Use only

Images

ABIN190847 (0.2µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Annunen, Helaakoski, Myllyharju et al.: "Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17342-8, 1997 (PubMed).