Neuroligin 2 (NLGN2) antibody

Antigen: Neuroligin 2 (NLGN2)

Synonyms: KIAA1366, NL2, MGC67386, RP23-422L16.24, Nlgn2, GB10066, NLGN2, LOC574368, CCE-L2, NV50031, Nv123771

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190848

Additional Information

Alternative name: Neuroligin 2

Gene ID: 57555, 216856 (mouse), 117096 (rat)

Immunogen: C-NPPDTDIRDPGKK

Format: Affinity-purified

Description: All names and symbols: neuroligin 2 , KIAA1366, NLGN2. Official Symbol: NLGN2. RefSeq number(s): NP_065846.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 90kDa band observed in Human Pancreas lysates (calculated MW of 90.8kDa according to NP_065846.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology

Restrictions: For Research Use only

Images

ABIN190848 (0.1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Ichtchenko, Nguyen, Suedhof: "Structures, alternative splicing, and neurexin binding of multiple neuroligins." in: The Journal of biological chemistry, Vol. 271, Issue 5, pp. 2676-82, 1996 (PubMed).