Immediate Early Response 5 (IER5) antibody

Antigen: Immediate Early Response 5 (IER5)

Synonyms: SBBI48, MGC102760, MGC136422, MGC173748, wu:fa99a02, wu:fb04b03, wu:fb11f08, zgc:136422, si:dz129i22.1, sbbi48, MGC83180, IER5

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190870

Additional Information

Alternative name: IER5

Gene ID: 51278

Immunogen: C-QPPSGGEDDDAEE

Format: Affinity-purified

Description: All names and symbols: IER5, immediate early response 5, MGC102760, SBBI48. Official Symbol: IER5. RefSeq number(s): NP_057629.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Preliminary experiments gave an approx 75kDa band in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.7kDa according to NP_057629.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Cirelli, Tononi: "Gene expression in the brain across the sleep-waking cycle." in: Brain research, Vol. 885, Issue 2, pp. 303-21, 2001 (PubMed).