Neurochondrin (NCDN) antibody

Antigen: Neurochondrin (NCDN)

Synonyms: KIAA0607, Ms10ae, norbin, AU042419, MMS10-AE, mKIAA0607, DmelCG2330, CG2330, Norbin, MGC93259, NCDN, ncdn, MGC128555, GB14786

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190873

Additional Information

Alternative name: Neurochondrin

Gene ID: 23154, 26562 (mouse), 89791 (rat)

Immunogen: C-HYLLQVGSEKQKEP

Format: Affinity-purified

Description: All names and symbols: NCDN, neurochondrin , KIAA0607. Official Symbol: NCDN. RefSeq number(s): NP_055099.1, NP_001014839.1, NP_001014841.1

Specificity: This antibody is expected to recognize isoform 1 (NP_055099.1, NP_001014839.1) and isoform 2 (NP_001014841.1). Human, Mouse, Rat 729,71

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave bands at approx 100kDa and140kDa in Human Brain (Cerebellum) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.9kDa according to NP_055099.1 and 77.2kDa according to NP_001014841.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Publications

Publications: Dateki, Horii, Kasuya et al.: "Neurochondrin negatively regulates CaMKII phosphorylation, and nervous system-specific gene disruption results in epileptic seizure." in: The Journal of biological chemistry, Vol. 280, Issue 21, pp. 20503-8, 2005 (PubMed).