G-Protein Signaling Modulator 2 (GPSM2) antibody

Antigen: G-Protein Signaling Modulator 2 (GPSM2)

Synonyms: LGN, Pins, 6230410J09Rik, gpsm2, MGC52811

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190875

Additional Information

Alternative name: GPSM2

Gene ID: 29899

Immunogen: C-NRLKGKKYKTNSSTK

Format: Affinity-purified

Description: All names and symbols: GPSM2, G-protein signalling modulator 2 (AGS3-like, C. elegans) , HGNC:29501, LGN, Pins, LGN protein. Official Symbol: GPSM2. RefSeq number(s): NP_037428.2

Specificity: Human, Dog 677 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in Human Brain (Cerebellum) and Human Liver lysates (calculated MW of 75.8kDa according to NP_037428.2). Recommended concentration: 0.01-0.03µg/ml. An additional band of unknown identity was also consistently observed at 26-28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN190875 (0.01µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Yasumi, Sakisaka, Hoshino et al.: "Direct binding of Lgl2 to LGN during mitosis and its requirement for normal cell division." in: The Journal of biological chemistry, Vol. 280, Issue 8, pp. 6761-5, 2005 (PubMed).