Apolipoprotein M (APOM) antibody

Antigen: Apolipoprotein M (APOM)

Synonyms: G3a, NG20, HSPC336, MGC22400, 1190010O19Rik, APOM, MGC128780, fb62d12, wu:fb62d12, g3a, ng20, hspc336, DKFZp469N144

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN190884

Additional Information

Alternative name: APOM

Gene ID: 55937

Immunogen: PRNQEACELSNN

Format: Affinity-purified

Description: All names and symbols: APOM, apolipoprotein M , G3a, HSPC336, MGC22400, NG20 , NG20-like protein, G3a, NG20. Official Symbol: APOM. RefSeq number(s): NP_061974.2

Specificity: Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190884 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Xu, Nilsson-Ehle, Ahren: "Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects." in: The Journal of nutritional biochemistry, Vol. 15, Issue 10, pp. 579-82, 2004 (PubMed).