Galanin Receptor 1 (GALR1) (C-Term) antibody

Antigen: Galanin Receptor 1 (GALR1)

Synonyms: GALR1, GALR, GALNR, GALNR1

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN190892

Additional Information

Alternative name: GALR1 (C Term)

Gene ID: 2587

Immunogen: C-HLSDTKENKSRID

Format: Affinity-purified

Description: All names and symbols: GALR1, galanin receptor 1, GALNR, GALNR1. Official Symbol: GALR1. RefSeq number(s): NP_001471.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 40kDa band observed in Human Heart lysates (calculated MW of 38.9kDa according to NP_001471.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN190892 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Henson, Neubig, Jang et al.: "Galanin receptor 1 has anti-proliferative effects in oral squamous cell carcinoma." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22564-71, 2005 (PubMed).