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CCKA Receptor (mouse) antibody

Antigen

CCKA Receptor (mouse)

Clonality Polyclonal
Host

Goat

Reactivity

Rat (Rattus)

Application
ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN190898
Quantity 100 ug
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Gene ID 12425 (mouse), 24889 (rat)
Immunogen C-KFDASQKKSAKEKR
Format Affinity-purified
Description All names and symbols: Cckar, cholecystokinin A receptor , AW106902. Official Symbol: Cckar. RefSeq number(s): NP_033957.1
Specificity Mouse, Rat, Rabbit

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data kindly provided by Dr. Morisset, Sherbrooke, Canada. Data accepted by International Journal of Endocrinology (in press). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov,51(11):1501-13, PMID: 14566022). Recommended concentration: 0.1-0.3µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Bi, Scott, Kopin et al.: "Differential roles for cholecystokinin a receptors in energy balance in rats and mice." in: Endocrinology, Vol. 145, Issue 8, pp. 3873-80, 2004 (PubMed).