Paired Box 8 (PAX8) (Internal Region) antibody

Details for Product No. ABIN190912
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Antigen
Synonyms PAX8, XPax-8, XPax8, pax-8, PAX-8, Pax-8
Epitope
Internal Region
(16), (9), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(86), (28), (27), (16), (12), (4), (1), (1), (1)
Host
Goat
(53), (27), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(77), (48), (17), (17), (16), (10), (7), (6), (4), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN190912
374.27 $
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Immunogen Peptide with sequence C-AQPGSDKRKMDD, from the internal region of the protein sequence according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.
Specificity This antibody is expected to recognise all reported isoforms (NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PAX8
Background Synonyms: PAX8, paired box gene 8, HGNC:8622, paired domain gene 8, paired box 8
Gene ID 7849
NCBI Accession NP_054698, NP_039246, NP_039245, NP_039247, NP_003457
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: In transfected HEK293 transiently expressing PAX8A a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Paired Box 8 (PAX8) (Internal Region) antibody ABIN190912 (0.5µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Castro, Rebocho, Soares et al.: "PAX8-PPARgamma rearrangement is frequently detected in the follicular variant of papillary thyroid carcinoma." in: The Journal of clinical endocrinology and metabolism, Vol. 91, Issue 1, pp. 213-20, 2006 (PubMed).

Validation Images
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