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Antibodies
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anti-Paired Box 8 (PAX8) (Internal Region) antibody
Paired Box 8 (PAX8) (Internal Region) antibody
| Antigen |
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| Synonyms |
MGC93376, PAX-8, PAX8, XPax8, pax-8, XPax-8, MGC147590, DKFZp469G1314 |
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Binding Site
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (65), Rat (Rattus) (35), Mouse (Murine) (31), Cow (Bovine) (24), Dog (Canine) (21), Pig (Porcine) (5), Rabbit (5), Cat (Feline) (1), Chicken (1), Fruit Fly (Drosophila melanogaster) (1), Xenopus laevis (1), Zebrafish (1)
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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Western Blotting (WB) (45), ELISA (39), Immunofluorescence (IF) (22), Immunohistochemistry (IHC) (18), Immunoprecipitation (IP) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN190912 |
| Quantity |
100 ug |
| Price |
374.27 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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PAX8
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Gene ID
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7849
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Immunogen
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C-AQPGSDKRKMDD
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Description
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All names and symbols: PAX8, paired box gene 8, HGNC:8622 , paired domain gene 8. Official Symbol: PAX8. RefSeq number(s): NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1
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Specificity
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This antibody is expected to recognise all reported isoforms (NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1) Human
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa + 40kDa bands observed in lysates of cell line HEK293 (calculated MW of 48.2kDa according to PAX8A, NP_003457.1 and 41.7kDa according to PAX8B, NP_039245.1). Recommended concentration: 0.5-1.5µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Castro, Rebocho, Soares et al.: "PAX8-PPARgamma rearrangement is frequently detected in the follicular variant of papillary thyroid carcinoma." in: The Journal of clinical endocrinology and metabolism, Vol. 91, Issue 1, pp. 213-20, 2006 (PubMed).
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Alternatives for antigen "Paired Box 8 (PAX8)", type "Antibodies"
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Hosts
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Rabbit (40), Mouse (20), Goat (9)
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Reactivities
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Human (65), Rat (Rattus) (35), Mouse (Murine) (31), Cow (Bovine) (24), Dog (Canine) (21), Pig (Porcine) (5), Rabbit (5), Cat (Feline) (1), Chicken (1), Fruit Fly (Drosophila melanogaster) (1), Xenopus laevis (1), Zebrafish (1)
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Applications
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Western Blotting (WB) (45), ELISA (39), Immunofluorescence (IF) (22), Immunohistochemistry (IHC) (18), Immunoprecipitation (IP) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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N-Term (5), C-Term (3), Internal Region (3), Center (2), AA 191-206 (1), Isoform 8c,Isoform 8d (1)
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Alternatives 
2013 BioMed Central Research Awards
