Piwi-Like 1 (Drosophila) (PIWIL1) antibody

Antigen: Piwi-Like 1 (Drosophila) (PIWIL1)

Synonyms: HIWI, MIWI, PIWI, MGC150073, ziwi

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN190916

Additional Information

Alternative name: PIWI / HIWI

Gene ID: 9271, 57749 (mouse)

Immunogen: C-SNRKDKYDAIKKY

Format: Affinity-purified

Description: All names and symbols: PIWIL1, HIWI, PIWI, piwi-like 1 (Drosophila), piwi (Drosophila)-like 1, homolog of Drosophila piwi. Official Symbol: PIWIL1. RefSeq number(s): NP_004755.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 98.6kDa according to NP_004755.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Sharma, Nelson, Brandt et al.: "Human CD34(+) stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi." in: Blood, Vol. 97, Issue 2, pp. 426-34, 2001 (PubMed).

Qiao, Zeeman, Deng et al.: "Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas." in: Oncogene, Vol. 21, Issue 25, pp. 3988-99, 2002 (PubMed).

Szakmary, Cox, Wang et al.: "Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation." in: Current biology : CB, Vol. 15, Issue 2, pp. 171-8, 2005 (PubMed).