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Antibodies
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anti-alpha-Fetoprotein (AFP) antibody
alpha-Fetoprotein (AFP) antibody
| Antigen |
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| Synonyms |
FETA, HPAFP, AFP, MGC128267 |
| Clonality |
Monoclonal (4A3) |
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Host
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Reactivity
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Human (193), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (24), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Application
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Western Blotting (WB) (83), Immunofluorescence (IF) (66), Flow Cytometry (FACS) (65), Enzyme Immunoassay (EIA) (54), ELISA (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (IHC) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Radioimmunoassay (RIA) (19), Immunoprecipitation (IP) (16), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (5), Lateral Flow (LF) (5), ELISA (Capture) (4), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (4), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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| Catalog no. |
ABIN191205 |
| Quantity |
1 mg |
| Price |
290.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Immunogen
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Hybridoma Clone s have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human α-fetoprotein.
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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4A3
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Specificity
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MAbs react with different determinants of human α-fetoprotein. MAbs do not cross-react with human albumin. IgG1 for MAb 5H7 IgG2a for MAb 4A3
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Application Notes
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We recommend the following pair in sandwich immunoassay (coating – conjugate): 4A3 – 5H7
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Buffer
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PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative
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Storage
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+ 4 °C
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Research Area
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Serum/Plasma Proteins
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Restrictions
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For Research Use only
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Alternatives for antigen "alpha-Fetoprotein (AFP)", type "Antibodies"
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Hosts
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Mouse (145), Rabbit (83), Goat (14), Sheep (3), Duck (2)
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Reactivities
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Human (193), Mouse (Murine) (46), Rat (Rattus) (31), Dog (Canine) (28), Pig (Porcine) (24), Cow (Bovine) (23), Horse (Equine) (17), Rabbit (17), Chicken (5), Sheep (Ovine) (4), Monkey (3), Fruit Fly (Drosophila melanogaster) (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Western Blotting (WB) (83), Immunofluorescence (IF) (66), Flow Cytometry (FACS) (65), Enzyme Immunoassay (EIA) (54), ELISA (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (37), Immunohistochemistry (IHC) (21), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Radioimmunoassay (RIA) (19), Immunoprecipitation (IP) (16), ELISA (Detection) (5), Immunoelectrophoresis (IEP) (5), Lateral Flow (LF) (5), ELISA (Capture) (4), Immunodiffusion (ID) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (4), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2), Radial Immunodiffusion (RID) (2), Conjugation (1), ELISA (Coating) (1), Gel Diffusion (GD) (1), Indirect ELISA (iELISA) (1)
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Conjugates
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HRP (7), Biotin (6), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 647 (4), FITC (4), PE,Cy5.5 (4), Alexa Fluor 555 (3), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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Epitopes
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N-Term (3), a (2), Center (1), b (1), c (1)
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Alternatives 
